Hospital Regional de Alta Especialidad del Bajío, México
Title: Characterization of rhizospheric bacteria from soil around a lead recycling plant
Biography: M Maldonado-Vega
Pollution by fine dust from a recycling plant has an impact on living things. The adaptation of microorganisms is a biological alternative with a view to its biotechnological application in the treatment of industrial waste like the slags of a lead recycler. The present work was to isolate and characterize in vitro rhizospheric bacteria from surrounding soil to a Pb (NO3)2 tolerant lead recycling plant (Pb) and with capacity to produce indole compounds-indole acetic acid (AIA) and of sideróforos by means of the techniques of Salkowski and CAS-agar, respectively. We also identify bacterial isolates through the 16S rRNA gene. Ten strains tolerated concentrations between 0.6 and 1.8 mM Pb (NO3)2, of which seven strains had AIA production of up to 60 μg mL-1 and five strains had siderophore production of 0.02 to 0.054 μg mL-1. Qualitatively, the Streptomyces sp isolates presented a more intense orange halo than the Curtobacterium sp and Arthrobacter sp isolates, positive reaction indicator of siderophores production, similar to the positive control of Burkholderia cepacea. The production of siderophores of the hydroxamate type by the isolates L2, L48, L67, L138 and L23 was variable from 0.02-0.054 μg mL-1. The conclutions that there are bacterial strains associated to the rhizosphere of the vegetation cover around the recycling plant of Pb with a high potential to be used in phytoremediation processes. Tests of Ricinus communis plus inoculated with Streptomices sp and Bacillus sp. in industrial slag leave guidelines to address an alternative in industrial waste.